FastQCFastQC Report
Thu 2 Jun 2016
DRR028300_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028300_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17100.045.3559074
ATCAACG18250.043.2922173
CAACGCA19950.038.513165
GGTATCA18700.037.5990031
GTACATG45900.036.952971
TCTCGGG20250.034.72105145
GTATCAA25900.033.8634951
ACATGGG44500.033.7175873
TACATGG47500.033.114052
AACGCAG24200.032.348536
TACCTGG18950.029.8353772
ACGCAGA26700.028.2337277
CGCAGAG26100.028.0496238
ACCTGGG19200.027.5592773
CATGGGG30500.026.3796654
TATCAAC31200.025.7878152
CGCAGTA3709.094947E-1225.4675882
GAGTACA33900.025.4444711
ATAATAC13550.023.5374573
GTACCTG27250.022.07791
Produced by FastQC (version 0.11.5)