FastQCFastQC Report
Thu 2 Jun 2016
DRR028300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21550.043.060784
ATCAACG22600.041.3809553
CAACGCA23650.039.23725
GTATCAA27200.035.5396421
AACGCAG27050.035.1093866
GTACATG42600.034.635121
GGTATCA20250.033.739071
ACATGGG40450.031.5437533
TACATGG42300.031.1925052
CGCAGAG30700.030.6989978
TAGATCG1952.6101952E-729.7423025
ACGCAGA32150.029.3144387
TACCTGG19400.028.7745272
TATCAAC34600.027.4482352
ACCTGGG20850.026.4257163
GAGTACA30450.026.2564281
TATAACG2001.0664275E-525.3739012
GTACGGA2951.4300895E-824.5752076
CGCAGTA3301.7753337E-924.1656232
CATGGGG27100.023.8089334
Produced by FastQC (version 0.11.5)