FastQCFastQC Report
Thu 2 Jun 2016
DRR028299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989831
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22950.045.165454
ATCAACG24700.042.2589343
GGTATCA17850.042.232631
GAGCGAC7350.041.420467145
GTATCAA27400.039.417471
CAACGCA28100.037.1457565
GTACATG59900.036.5454371
AACGCAG30650.035.001316
TACATGG60600.033.731022
TCTCGGG17000.033.684586145
ACGCAGA31200.033.2226687
ACATGGG60450.032.495713
CGCAGAG31950.032.2159168
TATCAAC37600.028.3388712
GAGTACA42800.027.9442981
CATGGGG42800.023.7103144
GTACTAG6750.023.6250061
AGTACAT49550.022.235812
GCAGAGT44800.022.0046239
CCTAGTA9350.021.7069822
Produced by FastQC (version 0.11.5)