FastQCFastQC Report
Thu 2 Jun 2016
DRR028299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989831
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.050.8493841
TCAACGC30550.048.173664
ATCAACG31650.046.499383
GTATCAA34250.045.161121
CAACGCA33600.044.0165255
AACGCAG36400.040.8298036
GTACATG58000.036.4343221
ACGCAGA42150.034.2279057
CGCAGAG42100.034.096358
TATCAAC44450.033.761632
TACATGG62200.032.6356662
ACATGGG60050.031.7517433
GTACCGT1555.5042576E-528.0636656
GAGTACA43900.027.6247041
GCAGAGT53600.026.3751349
CATGGGG41200.024.9871064
GAGACGA5600.024.601177145
GTATACG2552.671246E-622.7821921
AGTACAT49650.021.9026582
TATACTG15900.020.0622865
Produced by FastQC (version 0.11.5)