FastQCFastQC Report
Thu 2 Jun 2016
DRR028298_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028298_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050736
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC14050.061.90702145
TCAACGC27300.045.9323234
ATCAACG27350.045.848353
GGTATCA20250.042.9527661
GTATCAA31600.042.663921
CAACGCA31900.039.536075
GTACATG60850.037.641021
AACGCAG34850.035.981426
ACATGGG58550.034.6629943
TACATGG60700.034.390522
ACGCAGA36900.033.786027
CGCAGAG36800.033.48398
TATCAAC41300.032.1170772
CATGGGG40250.029.3532814
CCGCGCG2603.7343852E-927.8779989
GCAGAGT47250.025.3114559
TTGTACG3352.1191227E-923.8003229
GAGTACA45500.023.7361261
CCGACGC7050.022.618746145
TCCGCGC2252.6581669E-522.5502038
Produced by FastQC (version 0.11.5)