FastQCFastQC Report
Thu 2 Jun 2016
DRR028298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050736
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG33950.048.047173
TCAACGC33750.047.9022754
GTATCAA35400.047.807261
GGTATCA23550.046.572251
CAACGCA35600.045.2093285
AACGCAG38850.041.240736
TATCAAC46600.035.0043222
CGCAGAG45900.034.116638
GTACATG62700.033.94231
ACGCAGA47300.033.5666547
ACATGGG58350.031.3101223
TACATGG63750.030.2500782
CATGGGG40050.027.152754
GCAGAGT57400.026.902519
TAGACCG1701.0320192E-425.5874735
GAGTACA47150.024.8019241
ATACCGT3551.546141E-1024.5063136
GTATAGG13200.022.5606251
TAGATCG3004.5610614E-721.7493525
TACCGTC2003.1052015E-421.7493527
Produced by FastQC (version 0.11.5)