FastQCFastQC Report
Thu 2 Jun 2016
DRR028297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718332
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22000.047.11414
ATCAACG22850.045.6787153
GTATCAA25550.043.404841
CAACGCA26350.039.6113365
GGTATCA18100.038.043691
AACGCAG29400.035.995086
CGCAGAG31350.033.2937368
GTACATG55300.033.0303341
ACGCAGA32300.032.763327
TATCAAC33050.031.58122
TACATGG54800.030.2895282
ACATGGG52500.029.8216743
GCGTATA1358.0960133E-426.8456441
GAGTACA41600.026.8327371
GACGAAA8500.026.435064145
CATGGGG37750.025.729154
GCAGAGT43750.024.1886929
GCCGACA4850.023.911995145
TAGACCG1650.002605107821.9646176
AGTACAT47000.021.7449742
Produced by FastQC (version 0.11.5)