FastQCFastQC Report
Thu 2 Jun 2016
DRR028297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718332
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27650.049.5556374
GGTATCA19950.049.1475071
ATCAACG28100.049.0200463
GTATCAA31350.045.6393361
CAACGCA30350.045.385945
AACGCAG34150.040.5479666
GTACATG56650.036.923531
CGCAGAG39250.033.9863558
TATCAAC40750.033.980692
ACGCAGA40800.033.228287
ACATGGG55000.033.2172973
TACATGG57300.033.0226822
CATGGGG38800.028.0276014
CGTGTCG3650.027.811523145
TAGATCG2156.1303217E-726.9760255
GCAGAGT50300.026.6642999
TAACCCG1100.00967474126.362935
GAGTACA41400.024.385131
GTACGGA3253.9952283E-822.3070966
TACGCTA1650.002602016321.969115
Produced by FastQC (version 0.11.5)