FastQCFastQC Report
Thu 2 Jun 2016
DRR028295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723818
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20200.047.3626174
CGACGAC6000.045.90347145
ATCAACG22200.043.42223
GTATCAA25150.041.4991
CAACGCA24350.039.290555
AACGCAG26050.036.7264866
GTACATG55950.036.6605951
TACATGG55650.034.3836442
GGTATCA16900.033.451921
TCTCGGG15500.033.20013145
ACATGGG55350.032.9986423
ATCTACC14400.031.709633145
ACGCAGA29450.031.5019767
GAGTACA40500.030.6023141
CGCAGAG29750.030.20988
TATCAAC35250.027.9635932
AGTACAT46350.025.3325212
CATGGGG40050.024.4311774
GCAGAGT41150.022.0167569
CGATCTC6200.021.042336145
Produced by FastQC (version 0.11.5)