FastQCFastQC Report
Thu 2 Jun 2016
DRR028295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723818
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25950.044.7000434
GGTATCA20300.043.2770421
GTATCAA28050.042.1913071
CAACGCA27900.041.8356975
ATCAACG28250.041.060753
AACGCAG30050.039.0837176
GTACATG55600.037.0861551
ACGCAGA35350.032.6086657
ACATGGG55650.032.568683
TACATGG57750.032.5142022
TAGATCG1801.2936471E-732.221285
CGCAGAG36200.031.6427218
TATCAAC40400.029.2503832
CTCCGAC4750.025.951881145
ACGACCG1701.0319216E-425.5874885
CATGGGG38600.025.167664
GAGTACA41400.024.2017631
CCGGTCG3052.03745E-823.7697989
GCAGAGT48650.023.247019
ACCGTCC2552.7096612E-622.7444328
Produced by FastQC (version 0.11.5)