FastQCFastQC Report
Thu 2 Jun 2016
DRR028294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794662
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22750.043.9689644
ATCAACG23850.041.9410443
GTATCAA27900.038.450861
CAACGCA26600.037.8775375
AACGCAG30350.033.6751066
GGTATCA20500.033.590641
GTACATG56150.033.5638771
ACGCAGA32450.031.2724487
CGCAGAG32250.030.5673488
TACATGG56900.030.4462762
ACATGGG55600.029.9848293
TATCAAC36250.028.3941482
GGACCGC2756.7884685E-926.3582066
GAGTACA41450.024.8320351
TGTACCG2252.6572845E-522.550915
CATGGGG38300.022.5214714
GCAGAGT43800.022.5067799
ACGATAG2303.1488198E-522.060673145
GTATTAG13850.021.4576741
GTATAGG12300.021.2151411
Produced by FastQC (version 0.11.5)