FastQCFastQC Report
Thu 2 Jun 2016
DRR028294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794662
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.054.5816151
TCAACGC31250.049.413754
ATCAACG31800.048.5591133
GTATCAA34200.046.7355651
CAACGCA34000.045.6302725
AACGCAG37250.042.2329756
ACGCAGA41800.036.5952267
CGCAGAG41250.036.5559128
TATCAAC43900.035.6703572
TAGACCG1051.8547982E-434.522245
GTACATG53850.034.2688031
TACATGG56050.029.2314972
ACATGGG54300.028.5714423
GCAGAGT52100.028.5255729
ACACCGT3451.09139364E-1025.2162426
GAGTACA41700.022.3010251
CATGGGG38550.021.4387134
GTGGTAT14100.020.6106551
AGTACAT43450.020.0221042
GTATAAA16400.019.9351541
Produced by FastQC (version 0.11.5)