FastQCFastQC Report
Thu 2 Jun 2016
DRR028293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729334
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC9950.075.03777145
TCAACGC21300.051.728494
CGACGAA5200.050.18396145
ATCAACG23400.047.086183
GTATCAA25550.044.8262061
CAACGCA26500.041.577995
GGTATCA16350.040.344971
AACGCAG28900.038.125156
GTACATG54350.034.5434721
ACGCAGA31050.034.3179637
CGCAGAG31850.033.2283828
TATCAAC34100.032.9490622
TACATGG55050.031.339022
ACATGGG52750.031.3312843
GAGTACA38400.028.5043721
TCTCGGG19950.027.614454145
CATGGGG38850.025.9351984
GTCGTAC2101.5579266E-524.1626451
GCAGAGT43100.023.8823389
TATACCG2151.8691617E-523.6007255
Produced by FastQC (version 0.11.5)