FastQCFastQC Report
Thu 2 Jun 2016
DRR028293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729334
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.052.7329861
TCAACGC27000.042.4244354
ATCAACG28600.040.3045273
GTATCAA29900.039.8439831
CAACGCA30050.038.118465
AACGCAG32500.035.4679876
GTACATG54400.034.8523181
ACATGGG51150.033.4494633
TACATGG54750.032.0445372
ACGCAGA37300.030.3206527
TATCAAC39050.030.075772
CGCAGAG38900.028.7007928
GAGTACA39550.025.8977891
CATGGGG37900.025.6323474
TTTCGCG1550.001809389623.3862769
TATCCCG2552.7098067E-622.74435
GCAGAGT48150.021.9826139
GTGGTAT14750.021.1771011
AGTACAT45150.020.7135582
TATATAG9400.019.2812395
Produced by FastQC (version 0.11.5)