FastQCFastQC Report
Thu 2 Jun 2016
DRR028292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333452
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15550.046.1490554
ATCAACG16050.044.7113883
CAACGCA18050.039.3556335
GTATCAA20150.037.052731
GTACATG36000.037.0487331
AACGCAG20500.035.005756
GGTATCA13250.033.3712121
TACATGG37700.032.3017432
GTACACG2507.094059E-1131.8941251
CGCAGAG22350.031.459538
ACGCAGA23050.030.5041477
ACATGGG38550.030.2732833
TATCAAC24450.029.6468932
TACACGG2354.3075488E-827.7608452
TCTCGGG9250.025.860102145
CATGGGG25550.024.9660474
TAGATCG1500.001496137224.1622185
GCAGAGT29250.023.790499
ACCTGGG12450.023.2888853
GTCTACG4358.367351E-1121.6626781
Produced by FastQC (version 0.11.5)