FastQCFastQC Report
Thu 2 Jun 2016
DRR028292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333452
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.055.196491
TCAACGC23400.048.3308334
GTATCAA25100.046.874771
ATCAACG24500.045.8649753
CAACGCA25200.044.590955
AACGCAG28050.040.577226
ATACCGA800.002037375236.2481276
TATCAAC33300.034.6153262
CGCAGAG33100.034.386448
ACGCAGA33050.034.219117
GTACATG38350.031.0582071
TACATGG39850.028.0161192
GTGGTAT11000.027.7302951
ACATGGG39000.027.325513
GCAGAGT41150.027.3072159
GAGTACA30300.024.928071
ATACGAC1851.8317538E-423.5122993
ACGGACC1902.188348E-422.903145
TAGCGTG1600.002176181922.6550795
GGGACCG1750.003660458420.7132155
Produced by FastQC (version 0.11.5)