FastQCFastQC Report
Thu 2 Jun 2016
DRR028291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621640
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20800.046.6962744
ATCAACG21900.044.6817743
GTATCAA23600.044.2273831
GAGCGAC5800.041.240765145
GGTATCA16450.039.6567731
CAACGCA26000.037.3570185
GTACATG50300.036.6021421
TAGATCG1656.0326784E-835.1436425
AACGCAG28350.034.2604036
TACATGG52600.032.6590352
CGCAGAG29050.032.187288
ACATGGG52800.031.7116473
TATCAAC31450.031.3443282
ACGCAGA30450.030.945457
GAGTACA37400.027.3267671
CATGGGG39750.024.9818254
GCAGAGT37900.024.4800059
AGTACAT42500.022.5126062
TCTCGGG11700.022.302698145
TACCGTC1650.002604935321.9647777
Produced by FastQC (version 0.11.5)