FastQCFastQC Report
Thu 2 Jun 2016
DRR028291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621640
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.056.064211
GTATCAA32550.055.0881771
TCAACGC35350.049.83564
ATCAACG35500.049.4208153
CAACGCA37350.046.9729235
AACGCAG39800.044.263536
TATCAAC45100.039.061832
CGCAGAG45950.037.5503778
ACGCAGA46650.037.2977337
GTACATG51000.036.297981
TACATGG52450.032.4821852
GCAGAGT54350.031.3466559
ACATGGG51700.030.5695323
TAGACCG1100.0096749726.3627175
CATGGGG39600.024.7150464
GAGTACA37500.023.4242971
CTATGCG1550.001809338923.386289
GTGGTAT12600.022.4701791
CAGAGTA53250.022.327869
GTATATA13150.022.0824221
Produced by FastQC (version 0.11.5)