FastQCFastQC Report
Thu 2 Jun 2016
DRR028290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345800
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC7800.058.541855145
TCAACGC18450.045.9631734
ATCAACG19150.044.2830543
GGTATCA13800.043.0680541
GTATCAA21700.042.4193951
CAACGCA21500.039.4428145
GTACATG40400.038.3930781
AACGCAG23750.035.7061276
TACATGG44250.033.087092
CGCAGAG26250.032.3055428
TATCAAC26700.032.3039932
ACGCAGA27100.031.5597237
ACATGGG46600.030.0187023
TATACCG1100.00968551526.3565045
GAGTACA31600.023.8543031
GCAGAGT33900.023.7325139
CATGGGG35500.023.683734
AGTACAT32050.022.6147842
TACACTG16700.020.8326855
AGAGTAC28350.020.7086815
Produced by FastQC (version 0.11.5)