FastQCFastQC Report
Thu 2 Jun 2016
DRR028290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345800
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.060.5135151
GTATCAA31150.049.1878661
ATCAACG30250.048.8908843
TCAACGC30500.048.490144
CAACGCA31950.046.2894945
AACGCAG35500.042.2731976
TATCAAC36300.041.1418382
ACGCAGA40550.037.0085957
CGCAGAG40850.036.559338
GTACATG40200.035.0436481
TACATGG44000.031.8000452
GTGGTAT10650.031.3647521
ACATGGG43750.030.3246733
GCAGAGT50300.029.2584389
GAGTACA32350.028.9566971
TAGATCG1409.998394E-425.8919685
ACGGACC2001.0642678E-525.379787145
AGTACAT34900.024.3043212
CATGGGG32900.022.476434
GTATAGG7800.022.3434521
Produced by FastQC (version 0.11.5)