FastQCFastQC Report
Thu 2 Jun 2016
DRR028289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968118
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.050.9709321
TCAACGC12950.045.337884
ATCAACG13400.043.815343
GCCGACA1701.8317223E-938.3762145
CAACGCA15600.037.6362535
GTATCAA16600.037.552281
AACGCAG16300.036.4646646
CGCAGAG17450.032.4000138
ACGCAGA18500.032.1283267
GTACATG25050.032.118941
TATCAAC19750.030.4618972
ACATGGG25300.028.0770533
TACATGG26450.027.6784442
GACGAAA3255.0931703E-1126.764938145
TCTCGGG7950.025.530499145
GTTAACG2051.291813E-524.752129145
CATGGGG18250.024.6249164
GCAGAGT22150.024.2160859
ATCGTCG2101.5575564E-524.1615458
GTATAGA6650.023.9798811
Produced by FastQC (version 0.11.5)