FastQCFastQC Report
Thu 2 Jun 2016
DRR028289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968118
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.060.5908361
GTATCAA19900.050.380521
TCAACGC19200.050.2188154
ATCAACG19400.049.701093
CAACGCA20350.047.3808945
AACGCAG22050.044.0567326
TATCAAC24350.040.7884942
TATAACG1651.366061E-939.5434572
ACGCAGA24850.039.092597
CGCAGAG24900.038.7229428
GTACATG26800.038.493751
ATAACGC1951.70985E-1037.177613
ACGGACC1803.1905074E-936.261276145
TACATGG26800.035.707152
GTACACG1655.9078957E-835.2243271
ACATGGG25350.034.317793
GCAGAGT30950.031.1535159
CATGGGG16950.028.2286594
GTATAGA6600.026.4182451
GTACGGA1409.997308E-425.8915486
Produced by FastQC (version 0.11.5)