FastQCFastQC Report
Thu 2 Jun 2016
DRR028288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133619
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.051.0146061
ATCAACG19700.048.9364283
TCAACGC19800.048.689274
GGTATCA12900.047.7612881
CAACGCA23850.040.72525
GTACATG43250.038.882011
AACGCAG25250.038.18016
TATCAAC25900.038.061512
ACGCAGA27050.035.3715027
TACATGG46050.034.3141862
CGCAGAG29550.031.8884018
ACATGGG49450.030.6356323
CATGGGG37850.028.3427964
GAGTACA32550.028.0586261
GCAGAGT34050.026.8225659
GTGGTAT8950.025.106461
TCCGCGC1751.2576614E-424.8519278
AGTACAT35450.024.7409272
ACACCGT2651.379467E-724.6174746
CCTATAC5000.023.195133
Produced by FastQC (version 0.11.5)