FastQCFastQC Report
Thu 2 Jun 2016
DRR028288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133619
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.11017811098790688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.10638494944068509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.066.925211
GTATCAA31300.052.4181671
TCAACGC33600.046.821354
ATCAACG34550.045.7437633
CAACGCA35850.043.882775
GTGGTAT8750.041.4839061
AACGCAG37950.041.4544756
TATCAAC39750.040.1244432
GTACATG42450.037.794821
ACGCAGA42700.036.843037
CGCAGAG45050.035.0820668
TACATGG44650.034.259772
ACATGGG46350.032.8467983
GCAGAGT50250.030.874599
TGGTATC13350.027.6957022
CATGGGG34700.026.5336954
GAGTACA31000.026.4627191
AGTACAT32950.023.1024132
GTCTAGC2202.2062544E-523.0989931
GTATTAG8350.022.6050031
Produced by FastQC (version 0.11.5)