FastQCFastQC Report
Thu 2 Jun 2016
DRR028287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC3050.057.03678145
GGTATCA13750.047.971391
ATCAACG16100.041.4195633
TCAACGC16150.040.8425144
TCTCGGG9000.037.852882145
GTATCAA19100.036.0523721
GTACATG34700.035.0932351
CAACGCA19150.034.444215
AACGCAG20550.032.4503636
GTAACGC1153.1665535E-431.5148873
TACATGG36250.030.7933042
ACGCAGA22100.029.8464537
ACATGGG37850.028.9170953
CGCAGAG22600.028.8654088
TATCAAC23700.028.7490232
TAACGCC1050.007717311427.6130454
GTATACG1358.094045E-426.8460161
GTACACG1801.5228069E-424.1614131
CATGGGG27250.022.8757614
CCGGTCG1952.6190662E-422.3028459
Produced by FastQC (version 0.11.5)