FastQCFastQC Report
Thu 2 Jun 2016
DRR028287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.051.1886331
TCAACGC21250.048.7867134
GTATCAA22250.047.9715271
CAACGCA22550.046.2956775
ATCAACG23050.044.976913
AACGCAG24800.042.3877946
CGCAGAG28150.036.8283358
ACGCAGA28650.036.69177
GTACATG36600.036.3049851
TATCAAC29450.035.202642
TACATGG37250.032.5023422
ACATGGG36400.031.867143
ACGGACC2101.4373654E-831.078043145
GCAGAGT34750.028.3732179
ATACCGC1100.009673926.3628146
CATGGGG27150.025.6345624
TATAACG1450.001226826224.9992222
GAGTACA27800.022.9844471
GTGGTAT12200.022.0210531
TAACGCA2653.7821046E-621.8861124
Produced by FastQC (version 0.11.5)