FastQCFastQC Report
Thu 2 Jun 2016
DRR028286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205603
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.048.508031
TCAACGC17700.041.3605084
GTATCAA19700.039.737021
ATCAACG18700.039.1487163
CAACGCA20000.036.604055
GTACATG41600.036.0673941
TACATGG41100.034.0371552
AACGCAG22000.033.276416
ACATGGG39350.032.7878573
TATCAAC24400.030.3003832
ACGCAGA23700.029.9719857
CGCAGAG23400.029.117218
CATGGGG30550.025.3869364
ACGATAG1650.00260465621.964626145
GAGTACA28950.021.7825361
GCAGAGT30150.021.6367979
GTATATA10950.021.1823231
GTATAGG4954.7475623E-1019.036011
TCTCGGG13300.018.529556145
TACCCCG2751.244668E-418.4502875
Produced by FastQC (version 0.11.5)