FastQCFastQC Report
Thu 2 Jun 2016
DRR028286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205603
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.062.3864971
GTATCAA25050.050.453551
TCAACGC26600.045.2425734
ATCAACG26900.044.738013
CAACGCA29300.041.0734635
AACGCAG31300.038.680586
TATCAAC33950.035.874872
ACGCAGA34900.034.2751547
CGCAGAG35550.033.444538
GTACATG38700.033.0332951
TACATGG38200.030.9346432
ACATGGG37250.029.777353
GCAGAGT42550.027.6017329
GTAGACG1100.009674218526.3625973
GAGTACA29350.024.0056691
GTGGTAT11550.023.8974631
CATGGGG26750.023.8495254
GTACGAC1550.001809119723.3861733
TAGACGT2603.206169E-622.306814
CAGAGTA40400.021.7132519
Produced by FastQC (version 0.11.5)