FastQCFastQC Report
Thu 2 Jun 2016
DRR028285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252814
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC5050.066.02789145
ATCAACG19400.046.7056353
TCAACGC19400.046.7056354
GTATCAA21250.044.6861951
GGTATCA13800.043.0720751
CAACGCA23600.038.3936165
TATCAAC25700.036.3845982
AACGCAG25500.035.5329136
GTACATG46150.034.7121541
CGACGAA3200.033.97835145
ACGCAGA27750.032.3906567
TACATGG47600.032.284192
CGCAGAG28150.031.9303958
ACATGGG47450.030.5530643
TCGCGTG950.004731849330.5209037
GAGTACA32600.028.4612121
GTATACG1658.430398E-526.3589631
CATGGGG33550.025.7107934
GCAGAGT35150.025.1591249
AGTACAT38500.022.7816752
Produced by FastQC (version 0.11.5)