FastQCFastQC Report
Thu 2 Jun 2016
DRR028285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252814
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.067.575261
GTATCAA28600.049.4966851
TCAACGC30150.045.926554
ATCAACG30200.045.8505173
CAACGCA32100.043.1366235
GTACATG46650.039.993461
AACGCAG35050.039.506016
TATCAAC37600.037.2123642
TACATGG48000.036.2483142
ACATGGG47250.035.749663
ACGCAGA38350.035.7284557
CGCAGAG39800.034.426798
GTATCCG950.004728823430.5248937
GAGTACA31250.030.1995771
GTGGTAT9500.029.8022141
CATGGGG33100.029.5681114
ACCGGAC1000.00607641628.998653
GCAGAGT47650.027.8423569
ACGGACC2559.4578354E-825.597261145
AGTACAT35550.024.8792922
Produced by FastQC (version 0.11.5)