FastQCFastQC Report
Thu 2 Jun 2016
DRR028284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146524
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.051.9189721
GTATCAA19100.043.2608761
TCAACGC18550.041.0269474
ATCAACG20050.037.95763
GTACATG38800.032.504341
CAACGCA23700.032.4176335
TACATGG40600.031.0632612
TATCAAC26300.030.3152242
ACGCAGA25450.029.6189337
ACATGGG41450.029.5519393
AACGCAG25850.029.4410026
CGCAGAG25500.028.42398
GGATAGG8000.027.180353145
TCTCGGG22050.026.62565145
TACCGTA1100.00968456726.3567057
GAGTACA28700.026.2648721
CATGGGG30850.025.8440954
GTATATA5350.025.7408941
GTATAAT7800.022.3018281
GTATTAA7850.022.1597791
Produced by FastQC (version 0.11.5)