FastQCFastQC Report
Thu 2 Jun 2016
DRR028284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146524
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT14690.12812640642498543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.057.7271731
TCAACGC22450.046.178794
ATCAACG23200.044.685943
GTATCAA23750.044.3486441
CAACGCA25200.041.139445
GTACATG41300.040.8050541
TACATGG40400.038.761022
AACGCAG27750.037.3590556
ACATGGG40750.036.8269353
TATCAAC29500.035.3885962
ACGCAGA32250.031.9213687
CATGGGG30050.031.36334
CGCAGAG33100.030.6635868
GAGTACA28250.029.3131451
CTCCGAC5350.028.461916145
TATATAG3351.8189894E-1228.1333475
GCAGAGT37700.026.9221389
AACCGTA1500.001494709824.1658257
AGTACAT32250.023.8286272
TCGATTA1952.5811457E-422.3507771
Produced by FastQC (version 0.11.5)