FastQCFastQC Report
Thu 2 Jun 2016
DRR028283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814949
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.040.4194681
GTACATG27400.039.681891
TACATGG26700.038.5503852
TCAACGC12600.038.5439224
GGTATCA9000.037.0481871
ATCAACG13250.036.1060263
GAGCGAC2252.0008883E-1135.437393145
CAACGCA14250.034.080945
ACATGGG29650.033.4924933
CATGGGG21150.031.8730934
AACGCAG15700.031.3950275
ACGCAGA16900.030.4525226
CGCAGAG17200.029.9213737
TATCAAC17850.027.2074721
GTGGTAT6450.025.8475721
GAGTACA19800.024.894041
GTACGGA1751.2571437E-424.8521986
GTATAGT5850.024.7813971
TACCTGG7050.024.6759432
TCTCGGG5900.024.571383145
Produced by FastQC (version 0.11.5)