FastQCFastQC Report
Thu 2 Jun 2016
DRR028283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814949
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.10896387381296253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.10381017707856564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.063.227771
GTATCAA23350.051.3052941
TCAACGC24050.047.6285254
ATCAACG24350.047.041733
CAACGCA26200.043.7200785
AACGCAG26750.043.3632056
TATCAAC29100.039.861362
ACGCAGA30300.037.564897
CGCAGAG32000.035.569268
GTACATG30350.035.405291
TACATGG31750.033.1092532
TATAACG1102.4361786E-432.953572
ACATGGG33100.031.1017993
GCAGAGT36700.030.6189869
GAGTACA22750.029.361051
GTACCGT1100.00967194926.3628566
AGTACAT25600.025.204332
CATGGGG21600.023.8303134
GTATAGA6400.023.8234391
GTATAAG5600.023.3372461
Produced by FastQC (version 0.11.5)