FastQCFastQC Report
Thu 2 Jun 2016
DRR028282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221417
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC8200.051.2699145
ATCAACG18100.051.260143
TCAACGC18100.051.260144
GTATCAA21050.044.420761
CAACGCA21500.042.8167465
AACGCAG23600.038.6996426
GGTATCA13350.036.9212151
TATCAAC25300.036.385772
GTACATG41750.035.765071
ACGCAGA26050.034.781697
CGCAGAG26050.034.781698
TAGATCG2008.971256E-932.6182675
TACATGG44050.031.4293792
ACATGGG45150.029.539863
GCAGAGT33450.026.8703869
GAGTACA33650.026.2798651
AGTACAT35300.023.4087642
CATGGGA16000.022.1985424
CATGGGG32650.021.9786194
CCGACGC4006.91216E-1021.74551145
Produced by FastQC (version 0.11.5)