FastQCFastQC Report
Thu 2 Jun 2016
DRR028282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221417
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.061.3464741
GTATCAA26200.045.4531561
TCAACGC25900.044.786574
ATCAACG26600.043.6079753
CAACGCA27700.041.6145255
AACGCAG31800.036.7050936
GTACATG44150.035.5258561
TATCAAC34500.034.673082
TACATGG44000.032.9537542
CGCAGAG35400.032.7675748
ACGCAGA35700.032.4922147
GAGTACA33150.029.3523431
ACATGGG46450.029.3426763
GCAGAGT41700.027.4693669
TACGACT1358.0848753E-426.8512084
AGTACAT35450.025.9725782
TAGATCG1550.001808970123.3865365
CATGGGG34800.023.3327734
GTGGTAT10800.022.187671
TCCGTAC1650.00260154821.969173
Produced by FastQC (version 0.11.5)