FastQCFastQC Report
Thu 2 Jun 2016
DRR028281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010330
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14150.042.514081
TCAACGC13150.041.88894
ATCAACG13700.040.2072263
GGTATCA10250.036.769741
GTACATG30500.036.3582271
CAACGCA15650.035.197385
AACGCAG16650.033.5187346
GCCGACA2900.032.4905145
TACATGG33050.031.5792752
ACATGGG31450.030.420363
TATCAAC19350.028.8417022
ACGCAGA19250.028.6150137
CGCAGAG19050.028.1545038
GTACACG1555.511439E-528.0563161
GACGAAA3453.6379788E-1227.31086145
TCTCGGG8850.027.026001145
TATAACG1100.00968541726.3559342
GAGTACA22500.024.8038621
GTCTCGC2101.5586118E-524.1596071
ATTATAC7900.023.853793
Produced by FastQC (version 0.11.5)