FastQCFastQC Report
Thu 2 Jun 2016
DRR028281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010330
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.053.9328351
GTATCAA21450.050.8163641
TCAACGC21800.048.5539554
ATCAACG21900.048.00123
CAACGCA22650.046.4117555
AACGCAG24400.043.677296
TATCAAC27950.037.8703462
ACGCAGA27950.037.3515747
CGCAGAG28300.036.889638
GTACATG31700.033.0097921
TACATGG32150.030.4425492
ACATGGG33150.029.9616173
GCAGAGT34900.029.4979049
AACACCG2207.4875607E-726.3630435
ACACCGT2001.0656693E-525.3744286
GAGTACA23250.024.6912861
CATGGGG24350.023.521034
GTATATA8750.023.2535671
CGCAGTA1600.002175449922.655742
GTACACG1952.574281E-422.3591981
Produced by FastQC (version 0.11.5)