FastQCFastQC Report
Thu 2 Jun 2016
DRR028280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988297
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1600.049.83594145
TCAACGC16300.047.1398544
GTATCAA16750.046.7389451
GGTATCA11500.046.6448631
ATCAACG16800.045.736883
CAACGCA19450.039.878075
AACGCAG20500.037.8355376
TATCAAC20500.037.4819342
ACGCAGA22250.035.1854977
GTACATG37450.033.6796381
CGCAGAG23800.032.284868
GGTTACG900.003630995832.2171752
TACATGG38700.031.6552472
CTTAGGG7350.028.600962145
GAGTACA28900.028.5941331
TATACTG5850.028.4998075
ACATGGG40750.028.4617983
GTATACA5250.026.2339841
GTGGTAT7250.024.9960841
AGTACAT31000.024.5526052
Produced by FastQC (version 0.11.5)