FastQCFastQC Report
Thu 2 Jun 2016
DRR028280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988297
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.10927889085973143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.10179126315267577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.060.1610951
GGTATCA16950.057.8444441
ATCAACG30000.052.198783
TCAACGC30350.051.8356864
CAACGCA31850.049.394455
AACGCAG33200.047.385946
TATCAAC34550.045.3245542
ACGCAGA38200.040.804027
CGCAGAG39300.039.6619228
GTACATG36950.036.559151
GCAGAGT45400.034.0135239
TACATGG39400.032.9370462
GTGGTAT8500.032.4685021
GAGTACA28750.031.0716881
ACATGGG42150.030.7881323
AGTACAT29550.029.931622
CATGGGG34000.024.7347154
CGTAGAC1902.1934905E-422.8942013
TGGTATC11800.022.1181282
CAGAGTA42850.020.641299
Produced by FastQC (version 0.11.5)