FastQCFastQC Report
Thu 2 Jun 2016
DRR028279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153820
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC4200.051.774803145
GGTATCA12450.045.9943241
TCAACGC15250.042.777874
ATCAACG15500.041.152623
GTATCAA18300.039.2130471
CAACGCA17400.037.49215
AACGCAG18350.035.156086
GTACATG34500.034.0363041
TCTCGGG8950.033.205296145
ACGCAGA20100.031.3739857
CGCAGAG20700.029.4140918
TACATGG34850.029.3266752
ATCTACC10550.028.16942145
TATCAAC24550.027.1633192
ACATGGG37650.026.3755823
GCGTATG1851.833521E-423.5085581
CATGGGG26650.023.1189544
GAGTACA25400.023.115211
TATTACG2252.6563192E-522.5508022
GCAGAGT27300.021.7719679
Produced by FastQC (version 0.11.5)