FastQCFastQC Report
Thu 2 Jun 2016
DRR028279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153820
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.053.7211881
TCAACGC20100.043.6425934
ATCAACG20400.043.0007933
CAACGCA20550.042.3341335
GTATCAA22650.039.758341
AACGCAG22450.039.7200746
GTACATG34450.033.7291561
TATCAAC26600.032.978052
ACGCAGA27350.032.6038637
TACATGG36100.031.730082
CGCAGAG28950.030.55158
ACATGGG37100.029.8977683
CTCCGAC3600.028.199469145
GCAGAGT33800.025.7386539
GAGTACA26250.025.7293221
CATGGGG26900.024.7945844
AGTACAT29000.022.249142
TACCTGG11500.022.0643672
GTGGTAT8200.021.2555391
CTATACT8350.019.969294
Produced by FastQC (version 0.11.5)