FastQCFastQC Report
Thu 2 Jun 2016
DRR028278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390188
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.046.9912571
GTATCAA22200.043.4266051
TCAACGC20550.042.6806224
ATCAACG21000.041.7660373
GTACATG48700.037.9549521
TACATGG48650.035.6100232
CAACGCA24850.035.0035485
TATCAAC27650.033.8183022
ACATGGG49650.033.4328543
AACGCAG26750.032.788296
ACGCAGA28800.030.4544037
CGCAGAG29450.029.53618
CATGGGG39750.025.7121964
GAGTACA37050.025.6295091
AGTACAT39850.024.010592
CGACGAA3052.0401785E-823.76607145
GCAGAGT36850.022.62139
GTGGTAT11050.020.991571
GTATACA8400.018.9845621
ACTAGAC7350.018.738013
Produced by FastQC (version 0.11.5)