FastQCFastQC Report
Thu 2 Jun 2016
DRR028278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390188
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.061.837271
GTATCAA28950.057.4744871
TCAACGC30700.052.188224
ATCAACG31350.051.1061673
CAACGCA33050.048.4774135
AACGCAG35850.044.8933946
TATCAAC36500.044.093922
ACGCAGA41900.038.238157
CGCAGAG42200.037.9663128
GTACATG47150.032.053081
GCAGAGT51900.030.3117499
TACATGG48500.029.297662
CGTATAC1306.485925E-427.883373
ACATGGG50650.026.9089663
GTATAGG6250.026.7384361
GTGGTAT12600.025.3729111
ACCGTCC1851.8317209E-423.5124638
CATGGGG37750.022.661244
GAGTACA38300.022.3857361
CTATACC6450.021.3556374
Produced by FastQC (version 0.11.5)