FastQCFastQC Report
Thu 2 Jun 2016
DRR028277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171668
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC5200.061.33429145
TCAACGC18150.047.123864
GTATCAA19450.046.9554941
ATCAACG18500.046.2323273
GGTATCA12950.043.6576461
CGACGAA3850.041.420563145
CAACGCA21650.039.5056885
GTACATG39650.038.5722271
AACGCAG23350.036.629476
TATCAAC24350.036.3158652
TACATGG41750.034.548732
ACGCAGA24750.033.9717757
CGCAGAG25850.032.8065728
ACATGGG43500.031.9924493
GCAGAGT31100.026.3362269
CATGGGG33450.026.0028294
TATACCG1400.001000939925.8867455
GAGTACA30100.025.5255361
TCTCGGG12250.025.44406145
CGTAATA1450.001228258424.9940992
Produced by FastQC (version 0.11.5)