FastQCFastQC Report
Thu 2 Jun 2016
DRR028277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171668
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.066.3996661
GTATCAA27200.050.7304461
ATCAACG29100.046.5877843
TCAACGC29200.046.4282384
CAACGCA30600.044.304075
AACGCAG32250.042.0373466
TATCAAC35250.038.8710372
ACGCAGA36250.037.198757
CGCAGAG36150.036.9005628
GTACATG39900.033.6730771
TACATGG41200.031.6736972
GCAGAGT45850.028.7776589
GAGTACA31700.028.4083751
ACATGGG45600.027.6635463
GTGGTAT9800.023.7141671
AGTACAT33950.023.7031822
TATACTG8800.022.243575
TCGCTCG1700.003093709521.3228159
CATGGGG34550.020.9833774
CATGGGA16300.020.4594364
Produced by FastQC (version 0.11.5)