FastQCFastQC Report
Thu 2 Jun 2016
DRR028276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504946
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.048.937841
TCAACGC21800.044.8865134
ATCAACG22450.043.2640383
GTATCAA24900.041.335921
CAACGCA25350.038.6006285
GTACATG47350.034.9022521
AACGCAG27850.034.8753176
TATCAAC32100.031.6126982
ACGCAGA30450.031.1833367
TACATGG51900.030.5854842
CGCAGAG30800.029.8876388
TCTCGGG12950.029.665024145
ACATGGG52250.028.1610163
GAGTACA36950.027.6594311
AGTACAT39950.023.4051552
TCTATAC10500.022.7804993
CATGGGG37250.022.3774264
GCAGAGT40100.022.0523099
TATACTG13200.020.8664345
CCGGTCG2455.1252333E-520.7095459
Produced by FastQC (version 0.11.5)