FastQCFastQC Report
Thu 2 Jun 2016
DRR028276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504946
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.056.8184551
GTATCAA31000.053.880171
ATCAACG32450.050.7151153
TCAACGC32650.050.182414
CAACGCA34250.048.0497975
AACGCAG36850.044.6595766
TATCAAC40600.040.891752
ACGCAGA42650.038.416317
CGCAGAG42350.038.3460738
GTACATG50650.034.9842951
ACATGGG52050.031.7570653
TACATGG53950.031.1761742
GCAGAGT51900.030.8710339
GAGTACA36250.027.6460971
AGTACAT39100.025.2167112
CATGGGA21100.023.3642984
CATGGGG37500.021.6527484
TATACAG12450.020.3809765
CAGAGTA48600.020.2875189
GTATAGG8600.019.4219191
Produced by FastQC (version 0.11.5)