FastQCFastQC Report
Thu 2 Jun 2016
DRR028275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068362
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16700.052.0864264
ATCAACG17350.050.5528563
GTATCAA19350.048.6992341
CAACGCA19250.045.563225
GGTATCA10950.042.36681
AACGCAG22350.039.5678186
TATCAAC22950.038.5333632
GTACATG35700.038.375441
CGCAGAG23350.037.8732648
ACGCAGA23550.037.5516247
GAGCGAC2900.034.995335145
TACATGG38050.034.6718142
ACATGGG40800.031.6242053
TACCGTC1652.3899847E-630.7520357
GCAGAGT28600.030.160659
CATGGGG29000.026.9951384
TTTCGCG1751.2573414E-424.8526659
GAGTACA27000.023.6253741
TACCTGG10850.022.71482
CATGGGA14450.022.5737884
Produced by FastQC (version 0.11.5)