FastQCFastQC Report
Thu 2 Jun 2016
DRR028275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068362
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.13862342539326558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.1187799640945672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.063.7610441
GTATCAA31300.056.8218541
TCAACGC34400.051.2120254
ATCAACG34700.050.769273
CAACGCA35400.049.5605665
AACGCAG37950.046.2304046
TATCAAC40350.043.839992
ACGCAGA43000.040.6324237
CGCAGAG43400.039.923848
GCAGAGT50200.034.2269949
GTACATG38750.033.720531
TATAACG1301.6552483E-533.460472
TATACCG1352.142806E-532.2211955
TACATGG40800.031.273512
ACATGGG41250.029.7020783
GAGTACA30250.025.1975061
CAGAGTA49350.024.6800619
CATGGGG28800.023.9141654
GTATAGA6100.023.8009221
TACCTGG10150.022.8564112
Produced by FastQC (version 0.11.5)